Hi,
We recently posted a blog where we measured the amber filter transmission. Here is the blog post: https://iorodeo.com/blogs/news/measuring-emission-spectra-for-the-blue-led-transilluminator

Below is a zoomed in version of the %Transmission graph so you can more clearly see transmission in the wavelengths of 400-550 nm.

The % transmission at 480 nm and 510 nm is about 1%. Transmission doesn't start to increase until around 525 nm approx.

So, this would probably not work for eGFP unfortunately.

Hi James,
I would recommend running the cyclic voltammetry experiment from 0.1 V to 1.0 V, at 50 mV/s for a few cycles (3-4 should be plenty). That would be a direct comparison to the experiment I described.
Let me know how that experiment works and we can go from there.
Jo

Hi James,
A couple of notes.

• In the ascorbate experiment described here, I used 50 mV/s, whereas you are using 500 mV/s (0.5 V/s)

• Also, in that experiment I was using ascorbate, so I think you can go ahead and start testing your set-up with ascorbate

• In my experience, the first CV cycle is different from the rest of the cycles. For example, when I run the experiment with 5 cycles, cycles 2-5 are very similar and overlap, whereas cycle 1 will be slightly offset from these. I usually use the cycles that overlap and discard data from cycle 1

Hi Jonas,
The colorimeter footprint is 15 cm x 9 cm. Height of the assembled colorimeter is 5.8 cm tall (or 6.2 cm with the rubber feet).
Hope that helps. Let us know if you have any other questions.
Thanks !

Hi, yes, for a second order, just place a new order on the online store. We would ship a new order in 2-3 days.

Hi horseflesh,
So, yes, you do need to do a calibration each time you start a new measurement.

The calibration measures the light intensity that reaches the sensor after passing through the cuvette and your blank sample -- which should not be absorbing any light due to the substance you are measuring e.g. nitrate.

The calibration is used as a reference when calculating the transmittance of light through your actual sample.

For the API nitrate test, a 'blank' sample would be 5 mL of distilled water (zero nitrate) developed with the test kit i.e. 10 drops of bottle 1, 10 drops of bottle 2. The color should be yellow like the image shown below. You would pour some of this into a cuvette and calibrate the colorimeter.

I hope that helps !
Jo

This is very interesting. Thank you for posting !

Hi Adrian,
The gel trays are plain acrylic, not solacryl (i.e. not UV transmissive). So they cannot be placed on a transilluminator. You would need to take the gel out of the tray and place it onto the transilluminator. The combs are Teflon.
Let me know if you have any follow-up questions.
Thanks !
Jo

Hi Adrian,
We have two wire options for the electrophoresis box. You can choose either stainless steel or platinum wire (+$40).
The platinum wire is a round soft wire 0.01" diameter.
Hope that helps,
Jo

Could you re-post your question and we will get back to you as soon as possible. Thanks !

So, with our gel trays, you use tape to close the ends of the tray. Most kinds of tape will work, just make sure you place tape over both ends and that it covers the edges well so it won't leak. You can test with some water if you want to check before pouring the agarose. Once the gel has set you can peel off the tape.