Thank you for your question. At this time we don't conduct teacher training workshops. However, we are always available to help and answer questions from individuals regarding using the colorimeter or any of our other products.
Posts made by Jo
RE: gel electrophoresis of DNA : failed!
Hi, I read through your message and I'm wondering whether this is a general question about gel electrophoresis or whether you are having trouble with your electrophoresis equipment. Are you using our IO Rodeo gel box and electrophoresis power supply ? It doesn't seem from your message to be an equipment problem, but more a problem with a new loading dye that you are using ? If that is the case, you might be better off asking the manufacturer of the loading dye whether you should expect to see you DNA sample behave differently in their buffer. I'm sorry I haven't used the GoTaq loading dye.
I hope that helps. Good luck with your PCR experiments and let us know if you have any specific questions about our electrophoresis equipment.
RE: Hi IO Rodeo folks
Thank you for your kind words about our work and for reaching out ! We will definitely take a look at the openhardware.science website, thanks. Your work sounds very cool, and we would love to chat further. We sent you a message on here so look for that and hopefully we can connect and chat soon !
RE: UV LEDs
We have looked into UV LEDs in the past for ethidium bromide, but unfortunately they are just too expensive.
I have not tried to do a sensitivity comparison for blue light vs. UV light for viewing ethidium bromide gels. I think that probably using a UV transilluminator will be the best option for ethidium bromide gels. We have made UV transilluminators in the past and have some instructions here: http://public.iorodeo.com/docs/uv_transilluminator/. We no longer sell the kit but maybe you can find something useful from the instructions. Otherwise, maybe you can find a supplier of inexpensive UV transilluminators.
Sorry we could not be more helpful.
RE: White transilluminator
We used the white light transilluminator with this Biotium DNAzure blue DNA stain:
I could imagine that this combination would work well in, for example, a classroom or educational setting as it doesn't require a dark room. I also did like that you can dry the gels and keep them which might be handy.
Other uses for the white light box might be for imaging Coomassie stained gels, for example.
However, I would recommend using the blue LED transilluminator for dyes such as GreenView, SYBRSafe etc.
At this time I haven't carried out a comparison of the sensitivity of the two dyes and transilluminators. You can find more information on the sensitivity of DNAzure on the Biotium link above.
I hope that helps !
RE: Parameters of LED Transilluminator amber filter
We recently posted a blog where we measured the amber filter transmission. Here is the blog post: https://iorodeo.com/blogs/news/measuring-emission-spectra-for-the-blue-led-transilluminator
Below is a zoomed in version of the %Transmission graph so you can more clearly see transmission in the wavelengths of 400-550 nm.
The % transmission at 480 nm and 510 nm is about 1%. Transmission doesn't start to increase until around 525 nm approx.
So, this would probably not work for eGFP unfortunately.
RE: Blue transilluminator
Below are some images of the green LED transilluminator we designed for a custom application using the superbright green LEDs I mentioned earlier. The enclosure is the same as the blue version, we just swap the 470 nm LED for the superbright green LED. A diffuser and green filter is placed over the LEDs as I mentioned.
The red viewing filter that sits above the LED board is actually a thin film Roscolux #26 filter which we laser cut.
Because it is thin, we have to support it. We can do this using a black frame.
Let me know what you think and whether this would work for your application.
We would be able to ship approx. a week after ordering. The lead-time would be on purchasing the LEDs from Superbright. Once we receive the LEDs we would ship 1-2 days later. The cost would be $225 for the fully assembled version with the 18V power supply. So it would be ready to use. I can send you a formal quote if you want to send me an email with your shipping info to email@example.com.
RE: Blue transilluminator
Yes, we can make custom LED boards for the LED transilluminator. You do want to find an LED that has a wide-viewing angle though. The one you have listed from Superbright has a 15 degree viewing angle, and we usually use LEDs with a 120 degree viewing angle. This will allow more uniform lighting. With the small viewing angle you can end up with bright spots.
We have made boards with this 530 nm LED from Superbright in the past: https://www.superbrightleds.com/moreinfo/through-hole/5mm-green-led-120-degree-viewing-angle-flat-tipped-1600-mcd/270/1197/
The actual spectra from this LED shows a peak at 518 nm so that might work ? If I recall correctly we used this in combination with a green acrylic filter (in place of the blue) and a red filter above the gel. I haven't tested with GelRed, but I think this could work.
RE: Power supply for gel electrophoresis australia
Hi, thanks for your questions.
Yes, that 15V power supply will work with our electrophoresis power kit.
Yes, we do ship to Australia. We would ship with DHL Worldwide.
Hope that helps,
RE: Mini gel box-- UK compatible?
@texas Yes, the mini gel box can be used in the UK. It is just the power supply that must be compatible, as you mentioned. If you already have an electrophoresis power supply that you use then it should work with our mini gel box.
Just fyi, our electrophoresis power supply kit works with a 15V wall adapter. The one we ship with the kit is this one from Digikey Part # 237-1435-ND. The input range for the 15V wall adapter is 90-260V AC so this will work in the UK. You would need a plug adapter though. You can also choose to supply your own 15V wall adpater with this kit.
Hope that helps !
RE: Use for aquaponics
So, the kit is just for making a small arduino-compatible colorimeter. It includes the hardware for making the device, which is a LED and sensor board, Arduino shield board and the enclosure hardware (plastic parts, screws, nuts etc.). There is open source firmware for the Arduino and software for acquiring data on your PC. Check out the documentation here: http://colorimeter.iorodeo.com.
For each aquaponics measurement you want to run with the colorimeter, you will need to supply your own reagents. We have carried out a few calibration tests for common aquaponics tests like ammonia, but I highly recommend that you make your own calibration curves with your own reagents.
Hope that helps !
RE: Extra large transilluminator
One option would be to use two of the large 14 cm x 14 cm LED arrays. If you place them side-by-side as shown in the image below, there is not much of a gap between the edges of the two arrays, and with the diffusers in place, this should give you an evenly lit large 28 x 14 cm area. This is larger than you need and you would need to use two 18V power supplies but it would get you up and running fairly quickly.
Designing a new LED array to the size you want is an option but would be more expensive as it would require designing the PCBs and having them manufactured. We are abit strapped for time at the moment and are not taking on any new projects at this time but we could certainly offer advice.
As far as the imaging enclosure, I did a quick check and the two large arrays just fit into the current design.
This is a top-down image showing the field of view:
Because it is such a tight fit, you could mount the LED boards, the diffusers, the blue filter and a clear acrylic cover for placing the gel directly into the imaging enclosure. This would be more of a permanent imaging enclosure set-up. We could help you set this up and design the larger parts you would need.
Hope this helps !
RE: Can the blue led transilluminators be used in Australia?
@jenny Yes, the transilluminators can be used in Australia. There is an option to select the correct power supply adapter including an Australian adapter option, so just be sure to select that option.
RE: Colorimeter Software for kinetic enzyme assay
This is a great question. A "Time vs. Absorbance" measurement is something we have been wanting to implement for exactly the type of kinetic assays you mentioned. On our end it is a matter of finding the time to work on this. It is very much on the list, so hopefully we will have this added sometime soon !
RE: Amber Acrylic Filter
Hi fingertips ! Yes, we recently ran into the same problem with the amber from McMaster-Carr. It seems that the manufacturer of the acrylic (Reynolds Polymer R-Cast acrylic I think) changed something with their material rendering it unusable for this application, as you mentioned. At the time we tried to get some information from the manufacturer, but we never heard back from them. In the meantime we started looking for alternatives and found a very similar amber which works so we have switched vendors now. I am sorry that we never got around to replacing the information on the website. I will look into doing that this week.
The amber we use now is from a local supplier. We usually order the material in bulk and pick-up from their shop. If you want we can send you some material to try. Please send me an email (firstname.lastname@example.org) with the size of material you are looking for and we will get some to you.
I was wondering also about whether you have tried cutting out bands with the amber placed at an angle ? The amber can be mounted in two ways, with one suitable for cutting out bands (see image below). Have you tried this ? I would be interested to in your feedback. Thanks !
RE: UV transparency
No, the gel tray and tray support section of the gel box are not UV transparent.
We have in the past made gel trays and the corresponding gel box support from Solacryl which is a "high transmisson" UV transparent material. Solacryl worked really well for viewing gels on our UV-B transilluminator so I would recommend it if you were looking for UV transmissive acrylic. I don't have a datasheet for the material though so I don't have a % transmission at 300-320nm. You could check with the company - Spartech - for a datasheet with % transmission.
RE: Additional tests for colorimeter (pH, alkalinity, calcium hardness, etc.)
@ebeuerle Hi Ed. Right now we are making some updates to the Colorimeter software to include pH as a measurement option and finalizing the calibration data. I hope to have this finished very soon so you can start measuring pH with your LaMotte indicator. Next up is alkalinity. I wanted to completely be finished with pH before staring a new test, but I think we are almost there.
Could you post here a link to the LaMotte test kits you use for alkalinity and calcium ? I will order those next. Thanks.